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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: A molecular study on recombinant pullulanase type I from Metabacillus indicus

Fig. 1

 A) 12% SDS-PAGE showing purification steps of Pull_Met using Nickel -affinity agarose chromatography. Lane M: Protein ladder. Lane 1: purified Pull_Met after Nickel -affinity agarose chromatography column. Lane 2: crude cell lysate of induced recombinant E. coli BL21 (DE3) Rosetta cells. B) 10% native-PAGE showing the multimerization status of Pull_Met. Lane M: Protein ladder. Lanes (1–4): Pull_Met purified to homogeneity using nickel affinity chromatography column.

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