Fig. 3From: An improved integrative GFP-based vector for genetic engineering of Parageobacillus thermoglucosidasius facilitates the identification of a key sporulation regulatorThe basic structure of the pMM7 spo0A deletion plasmid. It contains Gram-positive replication protein repB, and Gram-negative ColE1 origin, a kanamycin resistance cassette, the temperature-optimised sfGFP gene, and a region containing the homologous flanks that match the upstream and downstream regions of the spo0A target siteBack to article page