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Table 2 Comparison of dry cell weight, titer and yield of C30 carotenoids produced by engineered B. subtilis strains and relative increase compared to the control strain

From: Metabolic engineering of Bacillus subtilis toward the efficient and stable production of C30-carotenoids

Strain

DCW (g/L culture)

Titer Carotenoids (mg/L culture)

Yield Carotenoids (mg/g DCW)a

Relative increaseb

BsMN0

1.36 ± 0.09

1.01 ± 0.08

0.74 ± 0.07

1

BsMN1

1.23 ± 0.13

2.40 ± 0.13

1.95 ± 0.12

2.64

BsMN2

1.35 ± 0.05

2.96 ± 0.07

2.19 ± 0.08

2.96

BsMN3

1.43 ± 0.06

3.30 ± 0.11

2.31 ± 0.16

3.12

BsMN4

1.87 ± 0.16

4.22 ± 0.23

2.26 ± 0.32

3.05

BsMN5

1.32 ± 0.09

4.49 ± 0.19

3.39 ± 0.33

4.58

BsMN6

1.47 ± 0.08

6.51 ± 0.12

4.42 ± 0.19

5.97

BsMN6c

2.98 ± 0.14

9.11 ± 0.36

3.20 ± 0.24

NAd

  1. a The total amount of carotenoids was measured in triplicate (± standard deviation)
  2. b The relative increase is calculated as the amount of carotenoids produced in the engineered B. subtilis strain divided by the amount of carotenoids produced in the control strain (BsMNO) harboring the pHYCrtMN vector
  3. c Strain cultured in BS1 medium
  4. d Not applicable
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