Table 2 Sequences of Stem loops and primers
From: Overexpression of miR-32 in Chinese hamster ovary cells increases production of Fc-fusion protein
Name of the gene | Sequence of oligonucleotides from (5’–3') |
|---|---|
miR-32-5p Stem loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACGCAACT |
miR-32-5p Forward | CGTCCGTATTCCACATTACTAAGT |
U6 Stem loop | GTCGTATCCAGTGCAGGGTCCGAGGTATTCGCACTGGATACGACAAAATATGG |
U6 Forward | GCAAGGATGACACGCAAATTC |
Universal Reverse | GTGCAGGGTCCGAGGT |