Table 1 Enzymatic kinetic parameters of the three mutant proteins

37.67 ± 3.55

2.22 ± 0.07

18,509.92 ± 4341.41

491.42

45.52 ± 9.41

1.96 ± 0.17

16,366.99 ± 977.41

359.56

31.38 ± 5.61

5.98 ± 0.41

49,874.79 ± 2292.87

1589.33

33.38 ± 5.67

3.78 ± 1.01

31,517.63 ± 1408.41

944.16

96.78 ± 6.09

3.97 ± 1.18

33,146.88 ± 7908.62

342.48

33.27 ± 2.31

3.77 ± 0.97

26,180.56 ± 6736.11

786.91

40.82 ± 3.56

4.33 ± 0.54

17,182.54 ± 2142.86

420.93

28.2 ± 4.41

2.54 ± 0.029

15,484.74 ± 175.08

549.1