Fig. 4

Macroscopic and microscopic photographs of wound healing. a Wound healing appearances in all control and treated (Gel-H.P or Gel-H.P.Cur/infected or non-infected) groups (Three mice in each group) on days 0, 3, 6, 9, 12 and 15 were recorded using a digital camera. All of the treatments were performed only once on wounding day (day 0) and the healing process was followed until day 15. b Wound closure rate (%) of 6 groups on the day 12 next to post wounding. Wound area was measured using Image J software and the ratio of the wound size on the 12th to the zero days was calculated (mean ± SD, * P < 0.05, ** P < 0.01, *** P < 0.001). c–n Histopathological evaluation of the wounds using H&E staining on day 15 post-wounding. c, d, e, f, g and h are the control group (Table 1, group 4, P. aeruginosa: infected with 100 µL of P. aeruginosa (0.5 McFarland) without any treatment), while i, j, k, l, m and n are the treated group (Table 1, group 6, PAO1 + Gel-H.P.Cur: infected with 100 µL of P. aeruginosa (0.5 McFarland) + treated with Gel H.P.Cur). EP epidermis, DE dermis, HYP hypodermis, SG sebaceous glands, yellow arrows: EP, DE or HYP, blue arrows: hair follicles, green arrows: blood vessels