Fig. 2

Characterization of CpG C274-Loaded Polymeric Nanoparticles. Transmission electronic microscopy was used to visualize CSNPs A and pDNA-CPG C274/CSNPs B with an average diameter of 25.93 0.98 nm and 79.21 ± 0.23 nm rspectively. CSNP nanoparticles C and pDNA-CPG C274/CSNPs D zeta sizer load diagrams. E Nanoparticle size measurement following storage by freezing at −20 °C for 1 month. F In vitro release kinetic of CpG from CpG-NP at pH 5 and pH 7. *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001. G Oligreen fluorescent test to determine the degradation of DNA at 16, 12, and 72 h. The results show no DNA degradation after 72 h. The highest amount of DNA was obtained after 72 h, which indicates the slow release of DNA from the nanoparticle