Table 2 List of primers (with sequences) used in cloning, confirmation of mltG gene deletion and qRT-PCR analyses
Function | Primer name | Forward sequence (5′ → 3′) | Reverse sequence (3′ → 5′) |
|---|---|---|---|
Cloning | mltG-up | TACTCGAGATGCTGTCTATGTCTTTATG | ATCTGCAGCATAACAGTTCGTGTTTCTT |
mltG-dw | ATGGATCCGATGATCTTTATTTTGTAGCC | ATGTCGACTATTTTCTACGTCTACGCTA | |
Kan | ATAGGAGGGATTTATATGAGCCATATTCAA | TTAGAAGAACTCATCCATGGATGTCTGGAG | |
Transformation analysis | mltG | ATACTCGAGATGAAAAAGGCTAAGCAATC | ATTGGATCCTTACGAATCACTGCTTGA |
qRT-PCR | fruA | AGCAGATCAAACTACAGAGCCTACTG | GGACTGCTCGCACCATCA |
gtfC | GGTTTAACGTCAAAATTAGCTGTATTAGC | CTCAACCAACCGCCACTGTT | |
clpA | TTTTGGGAGGCCTGTTGCT | TGGCAACGGAGGCAATAATC | |
spaP | GACTTTGGTAATGGTTATGCATCAA | TTTGTATCAGCCGGATCAAGTG | |
vic | TGACACGATTACAGCCTTTGATG | CGTCTAGTTCTGGTAACATTAAGTCCAAT | |
atpA | TCTGCCCGAGAAAGATCGA | GACCATTGTTGCGGATTCG | |
atpC | TGGAATGGGATCGGACTTTTT | TCCAACCCACGTAATTCAAAGG | |
ropA | GCGGTCGCTAATGCTGAAAT | CACGTTGGACCTCATCATGAA | |
comCD | ACAATTCCTTGAGTTCCATCCAAG | TGGTCTGCTGCCTGTTGC | |
ccpA | GCCAACTCATCCTCAGCAACA | GCGCAGCGTGTCATTAATTC | |
ftsX | GCAGCAAGATTTGGATTG | GCTTGGGTTGGTCTTATT | |
murE | TGCCGACCAGCCACATGATTTC | AGGCAGCAGCAACTGCATTTTC | |
murN | GAGGCTGGCAATCATAAA | CGCAGAGAATACAGTGATAA | |
gbpB | ATGGCGGTTATGGACACGTT | TTTGGCCACCTTGAACACCT |