Table 1 Summary of sequential purification of periplasmic L-ASNase from Pseudomonas sp. PCH199
Purification steps | Volume (mL) | Protein mg/mL | Total protein (mg) | Specific activity (U/mg) | Total activity (U) | Yield (%) | Purification fold |
|---|---|---|---|---|---|---|---|
Crude extract | 810 | 0.825 | 668.25 | 0.5 | 334.125 | 100 | 1 |
(NH4)2SO4 precipitation | 90 | 1.725 | 155.25 | 1.35 | 209.588 | 62.727 | 2.7 |
Anion exchange chromatography | 2 | 1.5 | 3 | 38.34 | 115.02 | 34.424 | 76.68 |
Size exclusion chromatography | 2 | 0.147 | 0.294 | 47.18 | 13.871 | 4.151 | 94.36 |