Fig. 5
Graphical representation of kinetic study of PCH199 L-ASNase. Determination of Km and Vmax of purified L-ASNase for L-asparagine by non-linear regression analysis of experimental steady-state data. a Plot of the reaction velocities (V) versus substrate concentration (S: 0.1– 3.0 mM) fitted to the Michaelis–Menten equation. b The corresponding Lineweaver–Burk plot (Km = 0.164 mM and Vmax = 54.78 U/mg) of L-ASNase catalyzed reaction