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Fig. 3 | AMB Express

Fig. 3

From: Biochemical characterization of extremozyme L-asparaginase from Pseudomonas sp. PCH199 for therapeutics

Fig. 3

SDS-PAGE analysis of purified PCH199 L-ASNase. Proteins were separated on 10% SDS-PAGE and stained with silver stain. Lane 1 (C), cell-free periplasmic extract; Lane 2 (AS), ammonium sulfate precipitation extract; Lane 3 (FT), flow-through; Lane 4 and 5 (E1, E2), eluted fractions of anion-exchange chromatography; Lane 6 and 7 (E3, E4), eluted fractions of size-exclusion chromatography; Lane 8 (M), protein molecular weight marker (kDa). Arrow indicates homogenous purified protein

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