Fig. 2From: Effects of down-regulation of ackA expression by CRISPR-dCpf1 on succinic acid production in Actinobacillus succinogenesWestern blot analysis of Cas proteins (M, 180 kD protein ladder; Cas9opt, cas9 codon-optimized for A. succinogenes; kCas9 from plasmid pKCcas9dO; Cpf1 from plasmid pDZLcas12a)Back to article page