Table 2 Lysis efficiency of digestive enzymes and their combinations against Candida albicans SC5314
From: Rapid preparation of Candida genomic DNA: combined use of enzymatic digestion and thermal disruption
Number of blastospores | Combination use | Digestive enzyme | Digestion time (h) | Dosagea |
|---|---|---|---|---|
5 × 107 | No | Lyticase | 5 | 200 U |
5 × 107 | No | Snailase | 5 | 1 040 μg |
5 × 107 | No | Zymolyase | 5 | – |
5 × 107 | No | Glucanase | 5 | – |
5 × 107 | Yes | Lyticase | 1 | 650 μg |
Yes | Snailase | 1 | 100 U | |
5 × 105 | Yes | Lyticase | 1 | 130 μg |
Yes | Snailase | 1 | 20 U |