High-yield production and purification of prebiotic inulin-type fructooligosaccharides

AMB Express

Table 1 Product amounts from I-FOS synthesis in a 10 L scale^a

^aThe product analysis with 800 g L⁻¹ sucrose was performed three times. Bioconversion reactions from sucrose to I-FOS were started by adding 6000 U L⁻¹ crude inulosucrase to prewarmed solutions of 800 g L⁻¹ sucrose with 25 mM sodium acetate buffer (pH 5.5) and 1 mM CaCl₂. The reactions were performed at 40 °C for 20 h. Sucrose and product concentrations were determined via isocratic HPLC using 58% [v/v] acetonitrile at a flow rate of 0.7 mL min⁻¹