Fig. 2
From: Homemade plasmid Miniprep solutions for affordable research in low-fund laboratories
Validation of the home made miniprep kit. A Plasmid miniprep using the home-made kit in comparison with commercial kit. Lanes (1 and 2): plasmid minipreps using the GeneDirex commercial kit. Lane (3) plasmid miniprep using our home-made kit with RNase. Lane (4): plasmid miniprep using our home-made kit with RNase inclusion bodies. Lane (5) homemade kit with half amount of RNase A. B Agarose gel electrophoresis of px48SpCas9 plasmid minipreps PCR products: Lane (1 and 2): PCR for plasmid minipreps made using the commercial kit. Lanes (3 and 4): PCR for plasmid minipreps made using the home-made kit. Lanes (5 and 6) Negative and positive controls. C Agarose gel electrophoresis for DNA ladder PCR amplified fragments. Lane (1): commercial DNA ladder. Lanes (2 and 10): The home-made DNA ladder (mixture of the PCR amplified fragments. Lanes (3 to 9): PCR amplification for each fragment 100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 800 bp and 1500 bp