Table 1 Characteristics of strain ALW1 and some type strains of closely related species of the genus Microbulbifer
Characteristic | ALW1 | JCM 17,800 | JCM 31,875 | JCM 12,187 | JCM 16,147 | DSM 28,920 |
|---|---|---|---|---|---|---|
Temperature for growth (°C) | ||||||
Range | 10–45 | 10–40 | 15–45 | 15–45 | 10–45 | 15–45 |
Optimum | 30 | 30–35 | 30–35 | 30–35 | 35 | 30–35 |
pH | ||||||
Range | 5.0–10.0 | 5.0–9.0 | 5.0–9.0 | 5.0–10.0 | 5.0–10.0 | 6.0–10.0 |
Optimum | 6.0–7.0 | 7.0 | 7.0–8.0 | 7.0 | 6.0–7.0 | 7.0–8.0 |
NaCl (%) | ||||||
Range | 2.0–10.0 | 2.0–8.0 | 2.0–14.0 | 2.0–10.0 | 2.0–10.0 | 2.0–8.0 |
Optimum | 2.0 | 2.0 | 2.0 | 2.0–4.0 | 2.0 | 2.0 |
API 50CH | ||||||
D-Galactose | – | – | – | – | – | – |
D-Glucose | – | – | – | – | – | – |
D-Mannose | – | – | – | – | – | – |
L-Rhamnose | – | – | – | – | – | – |
D-Cellobiose | – | – | – | – | + | – |
D-Maltose | – | – | – | – | – | – |
D-Lactose | + | – | – | – | – | – |
D-Melibiose | – | – | – | – | – | – |
D-Sucrose | – | – | – | – | – | – |
D-Trehalose | – | – | – | – | – | – |
API ZYM | ||||||
Alkaline phosphatase | + | + | + | + | + | + |
Lipase (C14) | w | w | w | w | + | w |
Valine arylamidase | + | + | + | + | + | + |
Cystine arylamidase | + | + | – | – | + | – |
Chymotrypsin | – | – | – | – | + | – |
α-Glucosidase | – | – | – | – | – | – |
N-Acetylglucosaminase | – | – | – | – | – | – |
API 20NE | ||||||
Nitrate reduction | – | + | – | – | – | – |
Aescin hydrolysis | + | + | + | – | + | + |
Gelatin hydrolysis | + | + | – | + | + | + |
Glucose utilization | + | + | + | + | – | – |
Mannose utilization | – | – | – | – | – | – |
D-Maltose utilization | + | + | + | + | + | – |
Malic acid utilization | – | – | + | + | + | – |
Others | ||||||
Oxidase | w | + | w | – | – | + |
Catalase | w | + | + | w | w | – |
Starch hydrolysis | + | + | + | + | + | – |
Quinone composition | Q-8 (100%) | Q-8 (100%) | Q-8 (100%) | Q-8 (100%) | Q-8 (100%) | Q-8 (100%) |
Major fatty acids | Summed feature 8 (C18:1ω7c/C18:1ω6c, 20.29%), iso-C15:0 (14.67%), Summed feature 9 (C16:0 10-methyl/iso-C17:1ω9c, 13.87%), Summed feature 3 (C16:1ω7c/C16:1ω6c, 12.07%) | iso-C11:0 3-OH (19.13%), iso-C15:0 (15.94%), Summed feature 9 (C16:0 10-methyl/iso-C17:1ω9c, 14.99%), iso-C11:0 (14.94%) | Summed feature 9 (C16:0 10-methyl/iso-C17:1ω9c, 25.92%), iso-C15:0 (18.51%) | iso-C11:0 3-OH (18.34%), iso-C15:0 (13.80%), Summed feature 9 (C16:1 10-methyl/iso-C17:1ω9c, 13.46%) | iso-C15:0 (18.76%), Summed feature 9 (C16:0 10-methyl/iso-C17:1ω9c, 17.70%), iso-C11:0 3-OH (12.74%), iso-C11:0 (10.08%) | iso-C11:0 3-OH (26.70%), iso-C15:0 (14.67%), iso-C11:0 (13.69%) |
Cell wall sugar components | Ribose, galactose | Ribose, glucose | Ribose | Ribose, glucose | Ribose, galactose, glucose, mannose | Ribose, glucose |
Polar lipid profile†| PG, PE, UAL 1–2, UPL, UL, GL 1–2, UPGL | PG, PE, UAL 1–4, UL 1–3, GL 1–2, UPGL 1–2 | PG, PE, UAL 1–2, UPL, UAPL, GL 1–2, UPGL 1–2 | PG, PE, UAL 1–4, UAPL, UL 1–4, GL 1–2, UPGL | PG, PE, UAL 1–2, GL 1–2, UPGL 1–2 | PG, PE, UAL, UAPL, GL 1–2, UPGL, UL 1–2 |