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Table 4 Effect of replacement of Moringa oleifera leaf on in vitro digestibility, total gas, methane, PF and MBP along with roughage and concentrate feed (70R:30C)

From: Impact of Moringa oleifera on rumen fermentation and methane emission under in vitro condition

Parameters

0%

5%

10%

15%

20%

30%

40%

SEM

P value

Total gas (mL/gDM)

146.43 ± 3.89c

151.41 ± 4.76bc

157.26 ± 0.80abc

163.68 ± 4.92ab

165.95 ± 4.62ab

168.48 ± 5.46a

171.32 ± 1.85a

2.42

0.02

IVTDMD (%)

62.64 ± 0.67e

64.25 ± 0.43e

66.56 ± 0.69d

69.58 ± 1.54c

73.19 ± 0.37b

74.52 ± 0.48ab

75.85 ± 0.16a

1.10

 < 0.001

IVTOMD (%)

64.69 ± 0.14c

66.25 ± 0.40c

69.89 ± 1.31b

71.01 ± 1.46b

74.07 ± 0.81a

75.07 ± 0.47a

75.68 ± 0.01a

0.93

 < 0.001

CH4 (%)

32.16 ± 0.15a

31.03 ± 0.24b

30.55 ± 0.25b

29.44 ± 0.35c

28.57 ± 0.30d

27.94 ± 0.31d

27.78 ± 0.25d

0.35

 < 0.001

CH4 (mL/100 mg)

8.12 ± 0.13a

7.80 ± 0.15ab

7.87 ± 0.29ab

7.44 ± 0.31bc

6.90 ± 0.17c

6.79 ± 0.18c

6.88 ± 0.09c

0.13

0.001

Acetate (mM)

49.05 ± 1.23c

52.62 ± 0.68bc

56.83 ± 2.10ab

58.53 ± 2.46ab

61.01 ± 2.35a

62.98 ± 3.36a

63.47 ± 1.19a

1.30

0.002

Propionate (mM)

16.62 ± 0.52c

17.36 ± 1.22bc

18.69 ± 0.28abc

19.25 ± 0.86ab

19.66 ± 0.45ab

20.11 ± 1.19a

20.73 ± 0.38a

0.39

0.02

Butyrate (mM)

9.99 ± 0.30

10.04 ± 1.04

11.45 ± 0.16

10.87 ± 0.61

10.04 ± 0.91

9.14 ± 1.16

9.42 ± 0.53

0.29

0.41

AP ratio

2.96 ± 0.15

3.05 ± 0.17

3.04 ± 0.12

3.04 ± 0.04

3.11 ± 0.17

3.14 ± 0.11

3.06 ± 0.02

0.04

0.97

MBP (mg)

46.61 ± 0.46d

47.82 ± 1.91cd

49.94 ± 2.95bcd

50.17 ± 3.28bcd

53.17 ± 1.35abc

55.33 ± 1.27ab

56.85 ± 0.11a

1.005

0.018

PF

3.90 ± 0.17

3.84 ± 0.20

3.92 ± 0.06

3.84 ± 0.17

3.91 ± 0.13

3.98 ± 0.09

3.81 ± 0.09

0.047

 

Ammonia N (mg/dl)

13.23 ± 0.48

12.65 ± 0.81

12.76 ± 0.83

12.29 ± 1.71

13.76 ± 0.56

11.97 ± 1.18

11.97 ± 0.49

0.33

0.821

  1. Means with different superscripts a, b, c,  d and e in the same row differ significantly (P < 0.05)