Fig. 3From: Recombinant acetylxylan esterase of Halalkalibacterium halodurans NAH-Egypt: molecular and biochemical studySDS-PAGE (10%) showing the recombinant expressed AXE-HAS10 during the stages of purification using Ni2+-affinity agarose matrix.: Lane M: protein ladder in kDa. Lanes 1 & 2 purified AXE-HAS10 after elution from the Ni2+ -affinity agarose matrix column using the elution buffer (50 mM Tris- HCl, pH 8.0, 500 mM imidazole) indicated by a sold arrowBack to article page