Skip to main content

Table 3 Substrate specificity of BcelFp on PPD- and PPT-type ginsenosides

From: Cloning and characterization of thermophilic endoglucanase and its application in the transformation of ginsenosides

Type

Substratea

C3

C6

C20

Relative Activity (%)

(mean % ± SD)b

PPD-

Rb1

Glu(1→2)Glu-

 

Glu(1→6)Glu-

68.18 ± 1.51

 

Rb2

Glu(1→2)Glu-

 

Arap(1→6)Glu-

27.27 ± 3.28

 

Rc

Glu(1→2)Glu-

 

Araf(1→6)Glu-

25.18 ± 0.73

 

Rd

Glu(1→2)Glu-

 

Glu-

100 ± 1.27

 

Rg3

Glu(1→2)Glu-

 

H-

NDc

 

GypXVII

Glu-

 

Glu(1→6)Glu-

ND

 

CO

Glu-

 

Arap(1→6)Glu-

ND

 

CMc1

Glu-

 

Araf(1→6)Glu-

ND

 

F2

Glu-

 

Glu-

ND

PPT-

R1

Xyl(1→2)Glu-

Glu-

 

ND

 

Re

Rha(1→2)Glu-

Glu-

 

ND

 

Rg1

Glu-

Glu-

 

ND

 

Rg2

Rha(1→2)Glu-

H-

 

ND

 

Rh1

Glu-

H-

 

ND

  1. a Final concentration of substrate was 1.0 mM
  2. b The activity against Rd was assumed to be 100%, and corresponded to a specific activity of 10.8 U/mg
  3. c Not detected, specific activity is not detected by the analytical methods used in this study