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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: DAR-PCR: a new tool for efficient retrieval of unknown flanking genomic DNA

Fig. 1

Schematic depiction of DAR-PCR. Primary PCR is performed using SSP1 and the ISA primer; and the secondary PCR is performed using SSP2 and SSP3. The bud heterologous to the known DNA is indicated by the up-ended arrow. The solid lines denote known sequences; Dotted lines denote unknown sequences. SHSC: slightly high-stringency (60℃) cycle; LSC: low-stringency (25℃) cycle; MSC: moderate-stringency (55℃) cycle; HSC: high-stringency (65℃) cycle. The PCR primers are indicated by numbered arrows, and their locations in relation to the relevant strand of genomic DNA are shown on top of the diagram in Step 1

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