Table 2 The variables and their levels used in central composite design
From: Indirect optimization of staphylokinase expression level in dicistronic auto-inducible system
Variable | Levels | ||||
|---|---|---|---|---|---|
− α | − 1 | 0 | 1 | + α | |
Self-induction Temperature | 23 | 25 | 31 | 37 | 39 |
Inoculation Load | 0.027 | 0.03 | 0.04 | 0.05 | 0053 |
Media | LB | TB | 2YT | 3 Levels | |