Fig. 4
From: Indirect optimization of staphylokinase expression level in dicistronic auto-inducible system
Radial caseinolytic assay for SAK activity measurement on 5% skim milk-agar plate. a shows the expression of SAK in the dicistronic SILEX system at optimized conditions for three clones (wells 1, 2, and 3 are replicates). b shows the positive and negative control for SAK activity [well 2: commercial SAK with plasminogen (As the positive control), and well 3: Commercial SAK without plasminogen (As the negative control)]