Table 2 The optimized culture condition(temperature, pH and culture time) of A. niger, T. harzianum and T. reesei and their corresponding enzyme activity(CMCase, FPA and β-glucosidase)
Aspergillus niger | Trichoderma harzianum | Trichoderma reesei | |
|---|---|---|---|
Culture temperature | 30 °C | 25 °C | 35 °C |
Initial pH | 6.0 | 6.0 | 4.0 |
Culture time (h) | 96 | 36 | 36 |
CMCase (IU/mL) | 3.68 ± 0.42 | 1.72 ± 0.2 | 3.12 ± 0.52 |
FPA (IU/mL) | 0.04 ± 0.01 | 0.08 ± 0.02 | 0.13 ± 0.02 |
β-glucosidase (IU/mL) | 8.44 ± 0.6 | 0 | 0 |