Fig. 8.

1 Cloning and expression results. a Isolated DNA fragments by electrophoresis gel; 1S, colony PCR, 600 bp amplified DNA fragments belongs to the CsgA-MFP3 hybrid gene; 2S, DNA ladder; 3S, PstI restricted sample, plasmid cleavage within the MFP3 gene made it standing very slightly above the backbone plasmid; 4S, backbone plasmid. b SDS-PAGE gel; 1, zero-hour* (negative control); 2, first hour; 3, second hour; 4, fourth hour; 5, sixth hour; 6, eighth hour; 7, marker; c Western blot gel; 1 W, zero-hour (negative control); 2 W, fourth hour; *, expression hour. 2 Surface topographies of the chimeric CsgA-MFP3 protein together with height distribution diagrams for individual pH values. A section of 25 μm² at a pH 9, b pH 7.4, c pH 5, and d pH 3