Table 1 Results of the purification of extracellular laccase from C. gallica
From: Green production of a yellow laccase by Coriolopsis gallica for phenolic pollutants removal
Purification procedure | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Purification fold | Percent recovery (%) |
|---|---|---|---|---|---|
Culture supernatant | 20150.00 ± 624.61 | 654.50 ± 13.06 | 30.78 ± 0.34 | 100.00 ± 3.10 | 1.00 ± 0.01 |
Ammonium sulfate precipitation (90%) | 18806.67 ± 358.27 | 537.49 ± 2.18 | 34.99 ± 0.81 | 93.33 ± 1.78 | 1.14 ± 0.03 |
Anion exchange (DEAE-FF) | 9691.67 ± 162.63 | 69.78 ± 0.14 | 138.88 ± 2.06 | 48.10 ± 0.81 | 4.51 ± 0.07 |
Gel-filtration (Sephadex G-75) | 9195.67 ± 89.10 | 48.71 ± 0.27 | 188.79 ± 2.88 | 45.64 ± 0.44 | 6.13 ± 0.09 |