From: A joint PCR-based gene-targeting method using electroporation in the pathogenic fungus Trichosporon asahii
DNA fragment
Length of homologous regions (bp)
Total transformants
Homologous replacement
(∆cnb1)
Efficiency (%)
(∆cnb1/total transformants)
Joint PCR product
1500
45
8
18%
DNA fragment 1a
2000
21
4
19%