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AMB Express
Fig. 5 | AMB Express

Fig. 5

From: Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses

Fig. 5

Detection of the immune activity of recombinant phages against adenovirus using phage ELISA. Recombinant phages prepared from 92 randomly selected clones were added to the microplates coated with adenovirus vector rAd26. The M13K07 helper phage was used as a negative control, and PBS was used as a blank control. The HRP/anti-M13 monoclonal antibody was used to detect the bound phage. The highest OD450 nm value of the recombinant phage was 1.133, and the lowest value was 0.052. The negative control was 0.07. Two positive clones with the highest OD450 nm value were selected and named sdAb 5 and sdAb 31

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