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Fig. 10 | AMB Express

Fig. 10

From: Preparation and identification of a single domain antibody specific for adenovirus vectors and its application to the immunoaffinity purification of adenoviruses

Fig. 10

The affinity constants of recombinant sdAbs 5 to adenovirus rAd26 were determined by competitive ELISA. Affinity constants were expressed as the reciprocal of the adenovirus titer at 50% of the maximum OD450 nm value. The results showed that the 50% of the maximum OD450 nm value was 0.41225, and it can be found that the adenovirus titer at this point was about 5 × 108 vp/ml. Therefore, the affinity constants of purified clone 5 sdAbs to adenovirus rAd26 were determined to be 2 × 10–9 by calculation

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