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AMB Express

Table 1 List of primers and probes used in the study

From: Detection of low-load Epstein-Barr virus in blood samples by enriched recombinase aided amplification assay

Primers and probes

Sequence 5′–3′

Primer length (bp)

Forward Primer

CTACCTGTGCCGCATGAAACTGGGCGAGACCGA

33

Reverse Primer

CATGTCACAGTAAGGACAGAGAAGTCTGGG

30

Probe

GAACACCTGAGCGTGGTGAAGCCTCTAACGC-[FAMdT]-[THF]-[BHQ1-dT]-CTGTCCACTCCGAA-C3-spacer

48

  1. BHQ, black hole quencher; C3-Spacer, 3′ phosphate blocker; FAM, 6-carboxyforescein; THF, tetrahydrofuran.
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