Fig. 2

Expression of 4-FP degradation pathway genes. a PCR amplified fragments using plasmid from BL-control or BL-fpd as the template (M, DL2000). The plasmids were extracted when OD₆₀₀ reached 0.6 after being cultured in LB medium; b Relative transcript level analysis of the exogenous genes in BL-fpd at different concentrations of inducers by qRT-PCR. The relative expression values of the genes (relative to the internal control 16S gene) were calculated by 2^–ΔCT = 2^{−[CT(target)−CT(16S)]}. inducer 1 (0.2 g/L final concentration of L-arabinose and 0.1 mM final concentration of IPTG), inducer 2 (2 g/L final concentration of L-arabinose and 1 mM final concentration of IPTG). Values are the mean ± SD of three replicates