Fig. 4

Biochemical characterization of C25GH19B. A The pH-activity profile of C25GH19B. The activity was determined in 50 mM citric—phosphate buffer (●) in the range of pH 3.5–7.0 and 50 mM phosphate buffer (■) in the range of pH 6.5–8.0. B The temperature-activity profile of C25GH19B. C Thermal inactivation of C25GH19B incubated at different temperatures for 15 min. The enzyme protein in 50 mM acetate buffer (pH 5.0) was incubated at various temperatures for 15 min and assayed for residual activity at 30 °C. The activity of the samples without incubation was considered to be 100%. D Thermal inactivation profile of C25GH19B at 40 °C. E The effect of divalent metal ions on the enzyme activity