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AMB Express
Fig. 1 | AMB Express

Fig. 1

From: A novel recombinant chimeric bio-adhesive protein consisting of mussel foot protein 3, 5, gas vesicle protein A, and CsgA curli protein expressed in Pichia pastoris

Fig. 1

Map of the pPICZα A-GvpA-Mfp3 (A) and pPICZα A-CsgA-Mfp5 plasmid construction (B). Gene sequences were inserted between EcoRI and XhoI, EcoRI and NotI restriction sites, respectively. Proof of the successful transfection and presence of the desired chimeric gene in yeast by PCR amplification (C): Mfp5- CsgA (Lane c), GvpA-Mfp3 (Lanes d, e, f), and DNA marker (Lane a)

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