Fig. 1

Schematic view of a miniprep assisting instrument assembly. A Centrifuge/mixer-based apparatus. B Two-part rotor. C F1-F7—steps of the DM miniprep protocol. Pre-set parameters for each step of device-mediated miniprep showing the different settings of parameters such as time and speed of centrifugation (F1, F3–F7), time and intensity of vibration for pellet resuspension (F2) and oscillation frequency of rotor motion at amplitude 45° for liquid mixing (F3). F3 is a step that combines the mixing of the solution by oscillation of the rotor with an oscillation frequency of 400 rpm (Mixer 5) and an amplitude of 45° for 10 s, after which the device automatically starts centrifugation. Amplitude of 45° for liquid mixing is a constant parameter in the DM miniprep