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Table 4 Comparison of real-time fluorescence LAMP, PCR, and culture results from 16 clinical specimens

From: Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Ureaplasma urealyticum

Detection of UU Culturea Sensitivity (95% CI) Specificity (95% CI) PPV (95% CI) NPV (95% CI)
Pos Neg Total
Real-time fluorescence LAMPb Pos 16 0 16 100% (79.4–100)16/16 NA 100% (79.4–100)16/16 NA
  Neg 0 0 0     
PCRc Pos 14 0 14 87.5% (61.7–98.5)14/16 NA 100% (76.8–100)14/14 NA
  Neg 2 0 2     
  Total 16 0 16     
  1. PPV positive predictive value, NPV negative predictive value
  2. aCulture was performed on UU medium. Pos indicates that a UU strainwas isolated, and Neg indicates that no UU strain was isolated
  3. bReal-time fluorescence LAMP reaction was confirmed using a real-time quantitative PCR analyzer (ABI 7500). Pos indicates that amplification occurred in the UU LAMP assay, and Neg indicates that amplification did not occur in the UU LAMP assay
  4. cPCR was confirmed using an electrophoretic analysis (C1000™ Thermal Cycler). Pos indicates that amplification occurred with the UU PCR assay, and Neg indicates that amplification did not occur in the UU PCR assay