Evaluation of the real-time fluorescence loop-mediated isothermal amplification assay for the detection of Ureaplasma urealyticum

AMB Express

Table 4 Comparison of real-time fluorescence LAMP, PCR, and culture results from 16 clinical specimens

Detection of UU		Culture^a			Sensitivity (95% CI)	Specificity (95% CI)	PPV (95% CI)	NPV (95% CI)
Detection of UU		Pos	Neg	Total	Sensitivity (95% CI)	Specificity (95% CI)	PPV (95% CI)	NPV (95% CI)
Real-time fluorescence LAMP^b	Pos	16	0	16	100% (79.4–100)16/16	NA	100% (79.4–100)16/16	NA
	Neg	0	0	0
PCR^c	Pos	14	0	14	87.5% (61.7–98.5)14/16	NA	100% (76.8–100)14/14	NA
	Neg	2	0	2
	Total	16	0	16

PPV positive predictive value, NPV negative predictive value
^aCulture was performed on UU medium. Pos indicates that a UU strainwas isolated, and Neg indicates that no UU strain was isolated
^bReal-time fluorescence LAMP reaction was confirmed using a real-time quantitative PCR analyzer (ABI 7500). Pos indicates that amplification occurred in the UU LAMP assay, and Neg indicates that amplification did not occur in the UU LAMP assay
^cPCR was confirmed using an electrophoretic analysis (C1000™ Thermal Cycler). Pos indicates that amplification occurred with the UU PCR assay, and Neg indicates that amplification did not occur in the UU PCR assay