Primers | Sequences (5′–3′)a | Function | Length (bp) | Restriction enzyme |
---|---|---|---|---|
tolA-A | CGCAGAGCTCATTATTGAGGTTTCCGGAGTA | Upstream flanking regions of tolA | 301 | SacI |
tolA-B | TCTCGGTTCCCAAAAAACTGT | Â | ||
tolA-C | ACAGTTTTTTGGGAACCGAGAATACTTTTCTTTATGGAAGTT | Downstream flanking regions of tolA | 322 | Â |
tolA-D | CGGGGTACCTACCGCTATTGCGTAAATCTG | KpnI | ||
tolA-E | AGGAGCGGTTGAAACAACTTG | Internal regions of tolA | 562 | Â |
tolA-F | CTGAGATCGCCAAGCAGATCG | Â | Â | Â |
tolB-A | CGCAGAGCTCAATGTGTCTTGCATATTAGCCTG | Upstream flanking regions of tolB | 305 | SacI |
tolB-B | CATATCTCCCATACCTGGGCCTG | Â | ||
tolB-C | CAGGCCCAGGTATGGGAGATATGTAATAATTAATTGATTACTAA | Downstream flanking regions of tolB | 269 | Â |
tolB-D | CGGGGTACCACTTGTCGAGATCGAAGTAAA | Kpn I | ||
tolB-E | TGCGTTATGCAGGTCATACCG | Internal regions of tolB | 415 | Â |
tolB-F | TGACCGGAGGCGAGATCCATA | Â | Â | Â |
tolR-A | CGCAGAGCTCCGTTTCTTGGCACGGTAGGCT | Upstream flanking regions of tolR | 314 | SacI |
tolR-B | GGCTTACCCCTTGTTGCTTTC | Â | ||
tolR-C | GAAAGCAACAAGGGGTAAGCCAGTCTGCGTCCCGTTGGCTTG | Downstream flanking regions of tolR | 320 | Â |
tolR-D | CGGGGTACCGTTGCAGCTTTTTACGCTCTT | KpnI | ||
tolR-E | AGGTCGTCGCGAACTTAAGTC | Internal regions of tolR | 351 | Â |
tolR-F | AGCGCTTTAATTATTTCATCG | Â | Â | Â |