The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis

AMB Express

Table 2 Primers used for PCR amplification and detection

Primers	Sequences (5′–3′)^a	Function	Length (bp)	Restriction enzyme
tolA-A	CGCAGAGCTCATTATTGAGGTTTCCGGAGTA	Upstream flanking regions of tolA	301	SacI
tolA-B	TCTCGGTTCCCAAAAAACTGT	Upstream flanking regions of tolA	301
tolA-C	ACAGTTTTTTGGGAACCGAGAATACTTTTCTTTATGGAAGTT	Downstream flanking regions of tolA	322
tolA-D	CGGGGTACCTACCGCTATTGCGTAAATCTG	Downstream flanking regions of tolA	322	KpnI
tolA-E	AGGAGCGGTTGAAACAACTTG	Internal regions of tolA	562
tolA-F	CTGAGATCGCCAAGCAGATCG
tolB-A	CGCAGAGCTCAATGTGTCTTGCATATTAGCCTG	Upstream flanking regions of tolB	305	SacI
tolB-B	CATATCTCCCATACCTGGGCCTG	Upstream flanking regions of tolB	305
tolB-C	CAGGCCCAGGTATGGGAGATATGTAATAATTAATTGATTACTAA	Downstream flanking regions of tolB	269
tolB-D	CGGGGTACCACTTGTCGAGATCGAAGTAAA	Downstream flanking regions of tolB	269	Kpn I
tolB-E	TGCGTTATGCAGGTCATACCG	Internal regions of tolB	415
tolB-F	TGACCGGAGGCGAGATCCATA
tolR-A	CGCAGAGCTCCGTTTCTTGGCACGGTAGGCT	Upstream flanking regions of tolR	314	SacI
tolR-B	GGCTTACCCCTTGTTGCTTTC	Upstream flanking regions of tolR	314
tolR-C	GAAAGCAACAAGGGGTAAGCCAGTCTGCGTCCCGTTGGCTTG	Downstream flanking regions of tolR	320
tolR-D	CGGGGTACCGTTGCAGCTTTTTACGCTCTT	Downstream flanking regions of tolR	320	KpnI
tolR-E	AGGTCGTCGCGAACTTAAGTC	Internal regions of tolR	351
tolR-F	AGCGCTTTAATTATTTCATCG