Spontaneous quorum-sensing hierarchy reprogramming in Pseudomonas aeruginosa laboratory strain PAO1

AMB Express

Table 1 Identification of genome mutations in the PAO1-z strain

Nucleotide change	Amino acid change	Mutation type	Encoding	Targeted gene	Locus tag	Product
A1558324→ATCG	52I→IV	3-bp insertion	CDS	lasR	PA1430	Transcriptional regulator
C22278→T	No change	SNP	CDS	tsaP	PA0020	T4P secretin-associated protein
C3582640→A	157P→Q	SNP	CDS	gtrS	PA3191	Glucose transport sensor
GCGCTGTCGCGCCTGCGCA2807724→G	Truncated protein	18-bp deletion	CDS	mexT	PA2492	Transcriptional regulator
C5036907- > G		SNP	Noncoding

Whole-genome re-sequencing (WGS) was performed with the PAO1-z strain. Short reads were mapped to the reference PAO1 genome (NC_002516.2). Genome mutations were identified relative to the PAO1-u strain