Fig. 3
From: Multifunctional alkalophilic α-amylase with diverse raw seaweed degrading activities
SDS–PAGE analysis of the target protein Amy2587. M: Protein marker; Lane 1: Whole-cell of recombinant Escherichia coli BL21 (DE3) cells harboring pET-30(a)-amy2587 before induction; Lane 2: Whole-cell of recombinant Escherichia coli BL21 (DE3) cells harboring pET-30(a)-amy2587 after induction; Lane 3: Supernatant of recombinant Escherichia coli BL21 (DE3) cells harboring pET-30(a)-amy2587 before induction; Lane 4: Supernatant of recombinant Escherichia coli BL21 (DE3) cells harboring pET-30(a)-amy2587 after induction; Lane 5: Precipitate of recombinant Escherichia coli BL21 (DE3) cells harboring pET-30(a)-amy2587 before induction; Lane 6: Precipitate of recombinant Escherichia coli BL21 (DE3) cells harboring pET-30(a)-amy2587 after induction; Lane 7: Purified Amy2587