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Table 5 Performance of McGlc and PcGlc in optimized transglycosylation reactions at low and high conversions with 2 in suspension in the presence of 2-phenylethanola

From: Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

  TOFc Productivityd STYe
Xb 0.2 0.7 0.2 0.7 0.2 0.7
McGlc 2.1 (6.1) 0.14 (0.6) 174 (353) 11 (32) 50 (101) 3.2 (9)
PcGlc 1.2 (3.6) 0.51 (1.9) 135 (284) 57 (149) 29 (61) 12 (32)
  1. aUsing an S/E ratio of 55′000 mol mol–1; 35 °C; initial concentration of 2: 300 mM. The quantification of quercetin and compound 6 enabled the determination of the total (i.e., hydrolysis and transglycosylation) and the transglycosylation-specific conversion, respectively. The data in parentheses were calculated based on quercetin as the reaction product
  2. bX: conversion of glycosyl donor 2
  3. cTurnover frequency: 104 mol of 6 or quercetin·(mole of catalyst·h)–1
  4. dCatalyst productivity: g of 6 or quercetin·(g catalyst·h)–1
  5. eSpace–time yield: g of 6 or quercetin·(L·h)–1