Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

AMB Express

Table 5 Performance of McGlc and PcGlc in optimized transglycosylation reactions at low and high conversions with 2 in suspension in the presence of 2-phenylethanol^a

^aUsing an S/E ratio of 55′000 mol mol^–1; 35 °C; initial concentration of 2: 300 mM. The quantification of quercetin and compound 6 enabled the determination of the total (i.e., hydrolysis and transglycosylation) and the transglycosylation-specific conversion, respectively. The data in parentheses were calculated based on quercetin as the reaction product
^bX: conversion of glycosyl donor 2
^cTurnover frequency: 10⁴ mol of 6 or quercetin·(mole of catalyst·h)^–1
^dCatalyst productivity: g of 6 or quercetin·(g catalyst·h)^–1
^eSpace–time yield: g of 6 or quercetin·(L·h)^–1