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Table 3 Performance of McGlc and PcGlc in optimized transglycosylation reactions at low and high conversions with dissolved 2 and 2-phenylethanola

From: Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

  TOFc Productivityd STYe
Xb 0.2 0.7 0.2 0.7 0.2 0.7
McGlc 13 6.9 1240 640 86 44
PcGlc 8.9 2.9 901 292 57 19
  1. aUsing a substrate to enzyme (S/E) ratio of 67,000 mol mol–1. Reaction conditions: 35 °C, 25% (v/v) DMSO, 100 mM initial concentration of 2; 200 or 397 mM of 2-phenylethanol for McGlc or PcGlc-based reactions, respectively. The quantification of quercetin and compound 6 enabled the determination of the total (i.e., hydrolysis and transglycosylation) and the transglycosylation-specific conversion, respectively
  2. bX: conversion of glycosyl donor 2
  3. cTurnover frequency: 104 mol of 6·(mole of catalyst·h)–1
  4. dCatalyst productivity: g of 6·(g catalyst·h)–1
  5. eSpace–time yield: g of 6·(L·h)–1