Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

AMB Express

Table 3 Performance of McGlc and PcGlc in optimized transglycosylation reactions at low and high conversions with dissolved 2 and 2-phenylethanol^a

^aUsing a substrate to enzyme (S/E) ratio of 67,000 mol mol^–1. Reaction conditions: 35 °C, 25% (v/v) DMSO, 100 mM initial concentration of 2; 200 or 397 mM of 2-phenylethanol for McGlc or PcGlc-based reactions, respectively. The quantification of quercetin and compound 6 enabled the determination of the total (i.e., hydrolysis and transglycosylation) and the transglycosylation-specific conversion, respectively
^bX: conversion of glycosyl donor 2
^cTurnover frequency: 10⁴ mol of 6·(mole of catalyst·h)^–1
^dCatalyst productivity: g of 6·(g catalyst·h)^–1
^eSpace–time yield: g of 6·(L·h)^–1