Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

AMB Express

Table 2 Acceptor specificities for transglycosylation reactions with 2 as rutinosyl donor using McGlc or PcGlc^a as a catalyst—comparison with AnRut^b

Acceptor	McGlc	PcGlc	AnRut
Methanol	−^c	−	+
Ethanol	−	−	+
n-Propanol	+ ^d	+	+
n-Butanol	+	+	+
Pentan-1-ol	+	+	n.d
Pentan-2-ol	−	−	+
Hexan-1-ol	+	+	n.d
Catechol	−	−	+
Geraniol	−	−	+
2-Phenylethanol	+	+	+
2-Azidoethanol	+	+	+
1,2-Hexanediol	+	+	n.d
3-Allyloxy-1,2-propanediol	+	+	n.d
Sodium azide^e	+	+	+

n.d. not determined
^aBased on results obtained by thin-layer chromatography. Reaction conditions: 47 °C, 1.8 mM 2, 0.12 µg mL^–1 enzyme, 50 mM citrate buffer at pH 4.0 (McGlc) or pH 5.0 (PcGlc), 10% (v/v) DMSO
^bSee Šimčíková et al. (2015) and Brodsky et al. (2020); including unpublished results
^cNo transglycosylation product detected
^dTransglycosylation product detected
^eSeeKotik et al. (2021)