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AMB Express

Table 2 Acceptor specificities for transglycosylation reactions with 2 as rutinosyl donor using McGlc or PcGlca as a catalyst—comparison with AnRutb

From: Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

Acceptor McGlc PcGlc AnRut
Methanol −c −  + 
Ethanol − −  + 
n-Propanol  + d  +   + 
n-Butanol  +   +   + 
Pentan-1-ol  +   +  n.d
Pentan-2-ol − −  + 
Hexan-1-ol  +   +  n.d
Catechol − −  + 
Geraniol − −  + 
2-Phenylethanol  +   +   + 
2-Azidoethanol  +   +   + 
1,2-Hexanediol  +   +  n.d
3-Allyloxy-1,2-propanediol  +   +  n.d
Sodium azidee  +   +   + 
  1. n.d. not determined
  2. aBased on results obtained by thin-layer chromatography. Reaction conditions: 47 °C, 1.8 mM 2, 0.12 µg mL–1 enzyme, 50 mM citrate buffer at pH 4.0 (McGlc) or pH 5.0 (PcGlc), 10% (v/v) DMSO
  3. bSee Šimčíková et al. (2015) and Brodsky et al. (2020); including unpublished results
  4. cNo transglycosylation product detected
  5. dTransglycosylation product detected
  6. eSeeKotik et al. (2021)
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