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Table 1 Kinetic constants for the hydrolysis of compounds 14 using McGlc or PcGlc as a catalyst

From: Two fungal flavonoid-specific glucosidases/rutinosidases for rutin hydrolysis and rutinoside synthesis under homogeneous and heterogeneous reaction conditions

Compound Kinetic constants McGlc PcGlc
1 kcat [s–1] 53.9 ± 1.9 30.3 ± 1.5
KM [mM] 0.04 ± 0.01 0.03 ± 0.006
kcat/KM [s–1 mM–1] 1348 1010
  kcat [s–1] 22.7 ± 0.5 39.3 ± 1.2
2 KM [mM] 0.12 ± 0.03 0.21 ± 0.06
  kcat/KM [s–1 mM–1] 189 187
3 kcat [s–1] 23.0 ± 1.7 9.3 ± 0.8
KM [mM] 4.8 ± 0.1 0.38 ± 0.09
kcat/KM [s–1 mM–1] 4.8 24.5
4 kcat [s–1] 69.1 ± 6.0 120 ± 13
KM [mM] 0.21 ± 0.09 0.29 ± 0.1
kcat/KM [s–1 mM–1] 257 414