Fig. 4

Bioconversion of 1,3-PDO into 3-HP by growing cells of Acetobacter sp. CIP 58.66 in a pH-based fed-batch, preceded by a first growth step on glycerol in batch mode. A Overall 1,3-PDO consumption. B Overall 3-HP production. C 3-HPA production and residual 1,3-PDO in the bioconversion broth. These bioconversions were launched after a 32 h-long growth on glycerol. Therefore t = 0 h on these graphs is equivalent to t = 32 h on Fig. 3. For each pH condition, n = 2. Depending on replicates, initial working volumes were estimated to be between 0.91 and 0.96 L, and final volumes between 0.83 and 0.91 L