Fig. 4

Effect of sample load
(10, 12, and 14% w/w) of crude extract (molecular weight: 3 MDa) on hyaluronic
acid (HA) (□) and protein () recovery in the bottom phase of polyethylene glycol (PEG)–citrate aqueous two-phase systems (ATPS); and on HA purity (◆). PEG molecular weight was 8000 g mol−1, TLL 43% (w/w) and VR 3.50. Bars represent the
sample mean ± SEM
of experimental triplicates. Bars that do not share the same uppercase (HA) or lowercase (protein) letter are significantly different (p < 0.05)