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AMB Express
Fig. 3 | AMB Express

Fig. 3

From: The combination of R2R3-MYB gene AmRosea1 and hairy root culture is a useful tool for rapidly induction and production of anthocyanins in Antirrhinum majus L

Fig. 3

Comparison of coloration and expression analysis of anthocyanin-related genes by PCR in normal roots (wild type transformed hairy roots with AR1193/pBI121), and transformed hairy roots with AR1193/pBI35S:ROS1-35S:DEL. A Normal root of 6-week-old seedlings on MS medium; B A negative control hairy root transformed with AR1193/pBI121 (P121) after 6 weeks of culture on MS + 200 mg·L−1 of cefotaxime; C Transformed hairy roots emerging from the hypocotyl segment after 3 weeks infection with AR1193/pBI35S:ROS1-35S:DEL; D Transformed hairy roots of with AR1193/pBI35S:ROS1-35S:DEL after 6 weeks culture on MS + 200 mg·L−1 of cefotaxime. E Analysis of AmCHS, AmF3H, AmDFR and AmANS expression levels from a transformed hairy root with AR1193/pBI121 and five transformed hairy root lines with AR1193/pBI35S:ROS1-35S:DEL by semi-quantitative RT-PCR. P121, A negative control hairy root transformed with AR1193/pBI121; RD1 to 5, Five transformed hairy root lines with AR1193/pBI35S:ROS1-35S:DEL

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