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AMB Express
Fig. 4 | AMB Express

Fig. 4

From: Sulforaphane inhibits self-renewal of lung cancer stem cells through the modulation of sonic Hedgehog signaling pathway and polyhomeotic homolog 3

Fig. 4

SFN regulates self-renewal of lung CSCs through SHH Signaling Pathway and PHC3. a–c The efficiency of siRNA to silence Shh gene were determined by Western blotting. The relative protein levels were quantified with Image J software. d, e SFN inhibited cell viability of siRNA-Shh group less than that of si-control group when SFN concentration was 6, 8, 10 and 12 μmol/L. f Cell numbers of siRNA-Shh cells are significantly less than that of si-control group. And the inhibitory effect of SFN on siRNA-Shh cells are significantly lower than that of si-control group. g–i The efficiency of siRNA to silence PHC3 gene were determined by Western blotting. The relative protein levels were quantified with Image J software. j, k SFN inhibited cell viability of siRNA-PHC3 group less than that of si-control group when concentration was 8, 10 and 12 μmol/L. l Cell numbers of siRNA-PHC3 cells are significantly less than that of si-control group. And the inhibitory effect of SFN on siRNA-PHC3 cells are significantly lower than that of si-control group. N = 3, Data are presented as mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001

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