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Table 4 Recombinant E. coli cell-catalyzed bioreduction of various ketones in Tween-20/ChCl:Lys-containing system

From: Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Substrate Product Substrate concentration (mM) Reaction system Yield (%) ee (%)
1-(4-Fluorophenyl)ethanone (R)-1-(4-Fluorophenyl)ethanol 100 PBS buffer 42.2  > 99.9
Tween-20/ChCl:Lys-containing system 87.0  > 99.9
1-[4-(Trifluoromethyl)phenyl]ethanone (R)-1-[4-(Trifluoromethyl)phenyl]ethanol 100 PBS buffer 88.5  > 99.9
Tween-20/ChCl:Lys-containing system 95.8  > 99.9
Ethyl acetoacetate Ethyl (R)-3-hydroxybutyrate 1000 PBS buffer 63.2  > 99.9
Tween-20/ChCl:Lys-containing system 76.4  > 99.9
3,5-Bis(trifluoromethyl)acetophenone (R)-[3,5-Bis(trifluoromethyl)phenyl]ethanol 1000 PBS buffer 69.1  > 99.9
Tween-20/ChCl:Lys-containing system 86.3  > 99.9
  1. Reaction conditions: 0.063 g (DCW)/mM E. coli cells, 15% (v/v) isopropanol, 0.6% (w/v) Tween-20, 4% (w/v) ChCl:Lys, 30 ºC and 200 rpm, reaction for 18 h