Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

AMB Express

Table 1 Screening of biocatalysts

Strain	Yield (%)	ee (%)	Configuration
Recombinant E. coli BL21(DE3)-pET28a(+)-LXCAR-S154Y^a	26.9	> 99.9	(R)
Cyberlindneras saturnus ZJPH1807 (CCTCC M 2019215)	35.4	92.9	(S)
Galactomyces geotrichum ZJPH1810 (CCTCC M 2019822)	32.7	87.5	(S)
Geotrichum candidum ZJPH1704 (CCTCC M 2017380)	48.6	89.9	(S)
Pseudomonas aeruginosa ZJPH1504 (CCTCC M 2016188)	15.6	66.7	(S)
AH-18-3^b	47.4	99.9	(S)
GS-19-5^b	32.1	99.9	(S)
SX-18-1^b	37.6	98.2	(S)
SD-18-6^b	35.5	92.2	(S)
HN-17-1^b	40.8	89.1	(S)

^aRecombinant E. coli BL21(DE3)-pET28a(+)-LXCAR-S154Y affording carbonyl reductase (LXCAR) gene from Leifsonia xyli HS0904 was acquired in our previous study (Wang et al. 2014)
^bIsolates were screened out from different soil samples