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AMB Express
Fig. 2 | AMB Express

Fig. 2

From: Effective asymmetric preparation of (R)-1-[3-(trifluoromethyl)phenyl]ethanol with recombinant E. coli whole cells in an aqueous Tween-20/natural deep eutectic solvent solution

Fig. 2

Effects of key reaction parameters on the (R)-MTF-PEL production with recombinant E. coli cells in PBS buffer medium. Reaction conditions: a Effect of initial buffer pH: 100 mM 3'-(trifluoromethyl)acetophenone, 15% (v/v) isopropanol, 10.57 g (DCW)/L recombinant E. coli cells, 30 ℃, PBS buffer, 200 rpm, 12 h. b Effect of temperature: 100 mM 3'-(trifluoromethyl)acetophenone, 15% (v/v) isopropanol, 10.57 g (DCW)/L recombinant E. coli cells, PBS buffer (pH 7.5), 200 rpm, 12 h. c Effect of the ratio of biocatalyst to substrate concentration: 100 mM 3'-(trifluoromethyl)acetophenone, 15% (v/v) isopropanol, various recombinant E. coli cells as biocatalyst, PBS buffer (pH 7.5), 30 ℃, 200 rpm, 12 h. d Time course of (R)-MTF-PEL production with recombinant E. coli cells in PBS buffer medium: 15% (v/v) isopropanol, PBS buffer (pH 7.5), 30 ℃, 200 rpm, 8.6 g (DCW) /L recombinant E. coli cells under 100 mM substrate concentration (Black circle), 17.6 g (DCW) /L recombinant E. coli cells under 200 mM substrate concentration (Black up-pointing triangle). e Effect of substrate concentration on the (R)-MTF-PEL production with recombinant E. coli cells: 15% (v/v) isopropanol, 0.086 g (DCW)/mM recombinant E. coli cells, PBS buffer (pH 7.5), 30 ℃, 200 rpm, 21 h

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