Fig. 3

Evaluation of transformation, chromosomal segregation and transcription. a The presence of transporter genes was verified with the help of primers A1-A2 (araE), B1-B2 (araFGH) and C1-C2 (araJ). The primers produced fragments of ~1.42, ~3.58 and ~1.18 kb, respectively. WT genomic DNA served as the negative control. b Chromosomal segregation was examined with the help of primers D1-D2. In the absence of uninterrupted neutral site 1, the primers failed to generate PCR products, whereas the WT genomic DNA serving as the positive control yielded a ~1.20 kb product. c The presence of araBAD gene set was verified with the help of primers E1-E2. The primers produced ~4.19 kb band. WT genomic DNA was used as the negative control. d Chromosomal segregation was verified with the help of primers F1-F2. In the absence of uninterrupted neutral site 2, the primers failed to generate any PCR product, whereas the WT genomic DNA included as the positive control yielded a ~1.94-kb product. (e) Transcription of the transporter genes was demonstrated with the help of primers A1-A2 (araE), B1-B2 (araFGH) and C1-C2 (araJ), represented as ‘Test’. The primers generated products of ~1.42, ~3.58 and ~1.18 kb, respectively. As the positive controls (+), transcription of petA (~0.99-kb) was examined in the cDNA templates. As the negative controls (-), amplification of the transporter genes from the corresponding RNA samples was evaluated. (f) Transcription of araBAD gene set was demonstrated with the help of primers E1-E2, represented as ‘Test’. The primers generated products of ~4.19 kb. As the positive controls (+), transcription of petA (~0.99-kb) was examined in the cDNA templates. As the negative controls (-), amplification of the catabolic gene set from the corresponding RNA samples was evaluated