Synthesis of ellagic acid glucoside using glucansucrase from Leuconostoc and characterization of this glucoside as a functional neuroprotective agent

AMB Express

Table 1 Central composite design matrix for the experiment and predicted responses for the synthesis of ellagic acid glucoside

Run no.	Coded levels			Ellagic acid glucoside synthesis (mM)
Run no.	X₁	X₂	X₃	Actual	Predicted
1	150	300	5.0	0.97	0.47
2	560	300	5.0	0.75	0.36
3	150	1000	5.0	2.88	2.27
4	560	1000	5.0	2.70	2.30
5	150	300	20.0	1.45	0.82
6	560	300	20.0	1.08	0.67
7	150	1000	20.0	2.76	2.12
8	560	1000	20.0	2.62	2.10
9	10.2	650	12.5	0.72	1.65
10	699.8	650	12.5	1.02	1.55
11	355	61.4	12.5	0.01	0.66
12	355	1238.6	12.5	2.58	3.38
13	355	650	0.1	0.21	0.83
14	355	650	25.1	0.13	0.96
15	355	650	12.5	4.09	3.47
16	355	650	12.5	3.10	3.47
17	355	650	12.5	3.49	3.47
18	355	650	12.5	3.27	3.47
19	355	650	12.5	3.83	3.47
20	355	650	12.5	3.27	3.47

Y = − 4.630 + 0.011X₁ + 0.008X₂ + 0.443X₃ + 0.0000004X₁² − 0.000008X₂² − 0.00004X₃² − 0.00001 X₁X₂ − 0.000004X₁X₃ − 0.016X₂X₃